Nanoscale Hub

Electron Microscopic (EM) Hub

1.0 Objective
1.1 Organisation
1.2 Technical portfolio
1.3 Project request procedure
1.4 Fees
1.5 Publications

1.0 Objective

The Nanoscale hub has been established since 2017, not taking into account earlier logistic and administrative efforts or investments. It provides technical and scientific support for projects that require ultrastructural analysis. These include scientific questions that can't be addressed by light microscopy techniques due to resolution limitations. Typical examples within SyNergy comprise the characterization of neuronal, organellar, myelin, fibril or blood vessel morphologies or their context within tissue. Electron Microscopic (EM) investigations provide additional, non-hypothesis-driven insights. Currently, model organisms include mouse, zebrafish and human biopsies. The hub provides service and training for the whole workflow of Transmission (TEM) and Scanning EM (SEM) as well as Volume SEM. Image analysis support can only be provided to a limited extent. Depending on the project extent and complexity, either the Nanoscale Hub staff executes the work or trains scientists of the requesting SyNergy research group. SyNergy projects are prioritized but external project requests are considered depending on their general technical or scientific impact and benefit for future cluster-internal projects. The Nanoscale Hub integrates contiguous technologies, e.g. by collaborating with the Microscale Hub for correlative studies (CLEM). The Hub staff arranges contacts to groups with EM techniques that are not covered within the Hub.

Please contact the Nanoscale Hub staff for project requests or further questions (martina.schifferer@dzne.de)top

1.1 Organisation

Location of the Nanoscale Hub in the DZNE

Name	Function	Location	Mail	phone
Martina Schifferer	Hub group leader	DZNE, 2G 108	martina.schifferer@dzne.de	089-4400-46487
Georg Kislinger	Technical Assistant	DZNE, 2G 148	Georg.kislinger@dzne.de
Birgit Kunkel	Technical Assistant	DZNE, 2G 148	birgit.kunkel@dzne.de

SyNergy Coordinators

Name	Institution
Martin Kerschensteiner	Institute of Clinical Neuroimmunology – Hospital and Biomedical Center of the Ludwig-Maximilians-University
Thomas Misgeld	Institute of Neuronal Cell Biology (TUM-NCB) German Center for Neurodegenerative Diseases (DZNE)
Mikael Simons	Institute of Neuronal Cell Biology (TUM-NCB) German Center for Neurodegenerative Diseases (DZNE)

Activities

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1.2 Technical portfolio

• Sample processing (chemical fixation, HPF)
• TEM (Transmission Electron Microscopy)
• SEM (Scanning Electron Microscopy)
• ATUM (Automated Tape Collecting Ultramicrotomy)
• FIB-SEM (Focused Ion Beam SEM)
• Correlative EM (correlation strategy, EM part)
• Negative contrasting for in vitro studies

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