Munich Cluster for Systems Neurology

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Neural labeling and manipulation by neonatal intraventricular viral injection in mic

STAR Protoc. 2022 Jan 10;3(1):101081. doi: 10.1016/j.xpro.2021.101081. PMID: 35059654; PMCID: PMC8760487.

Authors/Editors: Wang M, Misgeld T, Brill MS.
Publication Date: 2022


This step-by-step protocol provides a fast and easy technique to label and/or
genetically manipulate neural cells, achieved by intraventricular injection of viral
vectors into neonatal mice under ultrasound guidance. Successful injection of ad-
eno-associated viral vectors (AAV) induces neural transduction as fast as 3 days
post injection (dpi) in both the central and peripheral nervous systems. Virally
driven expression persists until early adulthood. The same setup enables injec-
tion of other viral vectors as well as intramuscular injection.


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