A fluorescence microscopy-based protocol for volumetric measurement of lysolecithin lesion-associated de- and re-myelination in mouse brain.
STAR Protoc. 2022 Jan 29;3(1):101141. doi: 10.1016/j.xpro.2022.101141. PMID: 35141565; PMCID: PMC8810560.
|Authors/Editors:||Bosch-Queralt M, Tiwari V, Damkou A, Vaculčiaková L, Alexopoulos I, Simons M.|
Lysolecithin injections into the white matter tracts of the central nervous system are a valuable tool to study remyelination, but evaluating the resulting demyelinating lesion size is challenging. Here, we present a protocol to consistently measure the volume of demyelination and remyelination in mice following brain lysolecithin injections. We describe serial sectioning of the lesion, followed by the evaluation of the demyelinated area in two-dimensional images. We then detail the computation of the volume using our own automated iPython script.