A macrophage-T cell coculture model for severe tissue injury-induced T cell death.
STAR Protoc. 2021 Dec 1;2(4):100983. doi: 10.1016/j.xpro.2021.100983. PMID: 34927092; PMCID: PMC8646263.
|Authors/Editors:||Zhu J, Cao J, Liesz A, Roth S.|
A key observation of tissue injury, such as stroke and burn, is a state of systemic immunosuppression characterized by loss of T cells and rise of infections. Here, we present an in vitro model for cell-cell interactions between innate (macrophages) and adaptive (T cells) immune cells. This protocol facilitates bone marrow-derived macrophages (BMDMs) and splenic T cells in a coculture model The procedure mimics injury-induced T cell death, which is driven by inflammasome activation in macrophages.
For complete details on the use and execution of this protocol, please refer to Roth et al. (2021).