The use of a laser for correlating light and electron microscopic images in thick tissue specimens
Methods Cell Biol. 2014;124:323-37. doi: 10.1016/B978-0-12-801075-4.00015-X
| Authors/Editors: | Bishop D, Nikic I, Kerschensteiner M, Misgeld T. |
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| Publication Date: | 2014 |
Abstract
Correlating images between light and electron microscopy is difficult especially in tissue specimens with a substantial z-dimension. To facilitate correlated light and electron microscopy (CLEM) in thick tissue specimens, we describe a basic method of using a femto-pulsed near-infrared laser to burn precise three-dimensional fiducial markers that circumscribe cells or regions of interest for easy identification between imaging methods. This rapid and reliable approach permits traditional fixation and avoids the use of electron-dense labeling methods that can obscure ultrastructural details. The versatility of the technique permits CLEM in a variety of tissue specimens to allow interpretation of highly resolved ultrastructural data in the more macroscopic and potentially dynamic context of light microscopy.
