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Control of gene editing by manipulation of DNA repair mechanisms

Mamm Genome. 2017 Apr 3. doi: 10.1007/s00335-017-9688-5. [Epub ahead of print]

Authors/Editors: Danner E, Bashir S, Yumlu S, Wurst W, Wefers B, Kühn R.
Publication Date: 2017

2017_04_danner

Abstract

DNA double-strand breaks (DSBs) are produced intentionally by RNA-guided nucleases to achieve genome editing through DSB repair. These breaks are repaired by one of two main repair pathways, classic non-homologous end joining (c-NHEJ) and homology-directed repair (HDR), the latter being restricted to the S/G2 phases of the cell cycle and notably less frequent. Precise genome editing applications rely on HDR, with the abundant c-NHEJ formed mutations presenting a barrier to achieving high rates of precise sequence modifications. Here, we give an overview of HDR- and c-NHEJ-mediated DSB repair in gene editing and summarize the current efforts to promote HDR over c-NHEJ.

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